Purified Rabbit anti-Cytokeratin 17 Antibody

SKU: DL90664A

Size 1: 100 ul

Synonyms : Keratin, type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R, D, Rb

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Cytokeratin 17

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 17. The exact sequence is proprietary.

WB description: Western blot analysis of Cytokeratin 17 expression in PC12 (A), HeLa (B), A431 (C), NIH3T3 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Cytokeratin 17 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Cytokeratin 17 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 3872

Gene ID (Mouse):

Gene ID(Rat): 287702

SwissProt (Human): Q04695

SwissProt (Mouse):

SwissProt (Rat): Q6IFU8

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.