Purified Rabbit anti-Cyclin D3 (pT283) Antibody

SKU: DL94839A

Size 1: 100 ul

Synonyms : G1/S-specific cyclin-D3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Cyclin D3 (pT283)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin D3 (pT283). The exact sequence is proprietary.

WB description: Western blot analysis of Cyclin D3 (pT283) expression in HepG2 UV-treated (A), K562 UV-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin D3 (pT283) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 896

Gene ID (Mouse): 12445

Gene ID(Rat): 25193

SwissProt (Human): P30281

SwissProt (Mouse): P30282

SwissProt (Rat): P48961

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.