Purified Rabbit anti-Cyclin D2 Antibody

SKU: DL96732A

Size 1: 100 ul

Synonyms : G1/S-specific cyclin-D2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Cyclin D2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human Cyclin D2

WB description: Western blot analysis of Cyclin D2 expression in SW480 (A), NIH3T3 (B), mouse brain (C), mouse lung (D), rat heart (E) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin D2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Cyclin D2 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 894

Gene ID (Mouse): 12444

Gene ID(Rat): 64033

SwissProt (Human): P30279

SwissProt (Mouse): P30280

SwissProt (Rat): Q04827

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.