Purified Rabbit anti-Cyclin D1 (pT286) Antibody

SKU: DL93362A

Size 1: 100 ul

Synonyms : BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Cyclin D1 (pT286)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin D1 (pT286). The exact sequence is proprietary.

WB description: Western blot analysis of Cyclin D1 (pT286) expression in MCF7 EGF-treated (A), rat muscle (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Cyclin D1 (pT286) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 595

Gene ID (Mouse): 12443

Gene ID(Rat): 58919

SwissProt (Human): P24385

SwissProt (Mouse): P25322

SwissProt (Rat): P39948

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.