Purified Rabbit anti-CRHBP Antibody

SKU: DL98058A

Size 1: 100 ul

Synonyms : CRFBP; Corticotropin-releasing factor-binding protein; CRF-BP; CRF-binding protein; Corticotropin-releasing hormone-binding protein; CRH-BP

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to CRHBP

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human CRHBP

WB description: Western blot analysis of CRHBP expression in rat brain (A) whole cell lysates.

IHC description: Immunohistochemical analysis of CRHBP staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of CRHBP staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 1393

Gene ID (Mouse): 12919

Gene ID(Rat):

SwissProt (Human): P24387

SwissProt (Mouse): Q60571

SwissProt (Rat): P24388

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.