Purified Rabbit anti-COPS3 Antibody

SKU: DL91294A

Size 1: 100 ul

Synonyms : CSN3; COP9 signalosome complex subunit 3; SGN3; Signalosome subunit 3; JAB1-containing signalosome subunit 3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, Mk, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to COPS3

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.

WB description: Western blot analysis of COPS3 expression in HeLa (A), SP2/0 (B), mouse liver (C), rat liver (D), NIH3T3 (E), HEK293T (F) whole cell lysates.

IHC description: Immunohistochemical analysis of COPS3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of COPS3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 8533

Gene ID (Mouse): 26572

Gene ID(Rat): 287367

SwissProt (Human): Q9UNS2

SwissProt (Mouse): O88543

SwissProt (Rat): Q68FW9

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.