Purified Rabbit anti-CAR Antibody

SKU: DL95029A

Size 1: 100 ul

Synonyms : CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive activator of retinoid response; Constitutive active response; Constitutive androstane receptor; CAR; Orphan nuclear receptor MB67

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to CAR

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CAR. The exact sequence is proprietary.

WB description: Western blot analysis of CAR expression in Jurkat (A), mouse heart (B), mouse brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of CAR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 9970

Gene ID (Mouse): 12355

Gene ID(Rat):

SwissProt (Human): Q14994

SwissProt (Mouse): O35627

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.