Purified Rabbit anti-c-RAF (pS289) Antibody

SKU: DL98776A

Size 1: 100 ul

Synonyms : RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH

Background: Rabbit polyclonal antibody to c-RAF (pS289)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-RAF (pS289). The exact sequence is proprietary.

WB description: Western blot analysis of c-RAF (pS289) expression in AML12 (A), CT26 (B), HEK293T (C), A549 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of c-RAF (pS289) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene ID(Human): 5894

Gene ID (Mouse): 110157

Gene ID(Rat): 24703

SwissProt (Human): P04049

SwissProt (Mouse): Q99N57

SwissProt (Rat): P11345

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.