Purified Rabbit anti-C/EBP beta (pT235) Antibody

SKU: DL93372A

Size 1: 100 ul

Synonyms : TCF5; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to C/EBP beta (pT235)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human C/EBP beta (pT235). The exact sequence is proprietary.

WB description: Western blot analysis of C/EBP beta (pT235) expression in HEK293T EGF-treated (A), COLO205 EGF-treated (B) whole cell lysates.

IHC description: Immunohistochemical analysis of C/EBP beta (pT235) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of C/EBP beta (pT235) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 1051

Gene ID (Mouse): 12608

Gene ID(Rat): 24253

SwissProt (Human): P17676

SwissProt (Mouse): P28033

SwissProt (Rat): P21272

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.