Purified Rabbit anti-Beta-catenin (pS33) Antibody

SKU: DL92885A

Size 1: 100 ul

Synonyms : CTNNB; Catenin beta-1; Beta-catenin

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D, P, Rb, S

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Beta-catenin (pS33)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Beta-catenin (pS33). The exact sequence is proprietary.

WB description: Western blot analysis of Beta-catenin (pS33) expression in HeLa (A), HepG2 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Beta-catenin (pS33) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Beta-catenin (pS33) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 1499

Gene ID (Mouse): 12387

Gene ID(Rat): 84353

SwissProt (Human): P35222

SwissProt (Mouse): Q02248

SwissProt (Rat): Q9WU82

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.