Purified Rabbit anti-ATRIP Antibody

SKU: DL92446A

Size 1: 100 ul

Synonyms : AGS1; ATR-interacting protein; ATM and Rad3-related-interacting protein

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, FC

Background: Rabbit polyclonal antibody to ATRIP

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATRIP. The exact sequence is proprietary.

WB description: Western blot analysis of ATRIP expression in MCF7 (A), HeLa (B), NIH3T3 (C), rat liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of ATRIP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ATRIP staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)

Gene ID(Human): 84126

Gene ID (Mouse): 235610

Gene ID(Rat):

SwissProt (Human): Q8WXE1

SwissProt (Mouse): Q8BMG1

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.