Purified Rabbit anti-APTX Antibody

SKU: DL96693A

Size 1: 100 ul

Synonyms : AXA1; Aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to APTX

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human APTX

WB description: Western blot analysis of APTX expression in A549 (A), K562 (B), Raji (C) whole cell lysates.

IHC description: Immunohistochemical analysis of APTX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of APTX staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 54840

Gene ID (Mouse): 66408

Gene ID(Rat): 259271

SwissProt (Human): Q7Z2E3

SwissProt (Mouse): Q7TQC5

SwissProt (Rat): Q8K4H4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.