Purified Rabbit anti-ALG-2 Antibody

SKU: DL96308A

Size 1: 100 ul

Synonyms : ALG2; Programmed cell death protein 6; Apoptosis-linked gene 2 protein; Probable calcium-binding protein ALG-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to ALG-2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human ALG-2

WB description: Western blot analysis of ALG-2 expression in SW620 (A), HepG2 (B), mouse kidney (C), mouse lung (D), rat ovary (E) whole cell lysates.

IHC description: Immunohistochemical analysis of ALG-2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ALG-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 10016

Gene ID (Mouse): 18570

Gene ID(Rat):

SwissProt (Human): O75340

SwissProt (Mouse): P12815

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.