POSTN ELISA kit (Human)

SKU: SEH339Hu

Size: 96Tests

# of Times Cited in literature: 34

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: POSTN

Target Full Name: Periostin

Alternative Names: OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2

Target Species: Human

Uniprot: Q15063

Gene ID: 10631

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human POSTN / Periostin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 78-5,000pg/mL

Sensitivity: 33pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Periostin (POSTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Periostin (POSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Periostin (POSTN). No significant cross-reactivity or interference between Periostin (POSTN) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periostin (POSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Periostin (POSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periostin (POSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Periostin (POSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Metabolic pathway;

References Citing This Product: Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients A Novel Marker Of Bone Strength?

Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY study

Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY Study

Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation

Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease

血清内脂素水平与多囊卵巢综合征的相关性研究

Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study

Serum periostin is a potential biomarker