Pig TNFb(Tumor Necrosis Factor Beta) ELISA Kit

SKU: ELK5858

Size:96T

Reactivity:Pig

Alternative Names:LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

Assay Type:Sandwich

Sensitivity:5.6 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Cytokine;Tumor immunity;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TNFb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TNFb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TNFb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TNFb in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P26445