Pig F1+2(Prothrombin Fragment 1+2) ELISA Kit

SKU: ELK6049

Size:96T

Reactivity:Pig

Alternative Names:F1+2

Assay Type:Sandwich

Sensitivity:2.6 ng/mL

Standard:400 ng/mL

Detection range:6.25-400 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Hematology

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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