Phospho-Vinculin (Tyr1133/Tyr1065) Antibody , Cat. # AF8406

SKU: AF8406

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:VCL

GeneID:7414

Uniprot:P18206

Specificity:Phospho-Vinculin (Tyr1133/Tyr1065) Antibody detects endogenous levels of Vinculin only when phosphorylated at Tyr1133(Isoform1,P18206-2) or Tyr1065(Isoform2,P18206-1).

Immunogen:A synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr1133/1065.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Horse,Sheep,Rabbit,Chicken,Xenopus

Application:WB,IF/ICC,ELISA(peptide)

Dilution:WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2840467

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF8406-BP

Des# Blocking Peptide:Phospho-Vinculin (Tyr1133/Tyr1065) Blocking peptide

Blocking Peptide Price:$549 CAD