Phospho-MTOR (Ser2448) Antibody - Cat. #: CSB-PA271384

SKU: CSB-PA271384

Size :100ul

Clone Number:

Aliases:dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody; FK506 binding protein 12 rapamycin associated protein 1 antibody; FK506 binding protein 12 rapamycin associated protein 2 antibody; FK506 binding protein 12 rapamycin complex associated protein 1 antibody; FK506-binding protein 12-rapamycin complex-associated protein 1 antibody; FKBP rapamycin associated protein antibody; FKBP12 rapamycin complex associated protein antibody; FKBP12-rapamycin complex-associated protein 1 antibody; FKBP12-rapamycin complex-associated protein antibody; FLJ44809 antibody; FRAP antibody; FRAP1 antibody; FRAP2 antibody; Mammalian target of rapamycin antibody; Mechanistic target of rapamycin antibody; mTOR antibody; MTOR_HUMAN antibody; OTTHUMP00000001983 antibody; RAFT1 antibody; Rapamycin and FKBP12 target 1 antibody; Rapamycin associated protein FRAP2 antibody; Rapamycin target protein 1 antibody; Rapamycin target protein antibody; RAPT1 antibody; Serine/threonine-protein kinase mTOR antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P42345

Immunogen:Peptide sequence around phosphorylation site of serine 2448 (T-D-S(p)-Y-S) derived from Human mTOR.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Background:

Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Albanell J,et al.(2007)Clin Transl Oncol.9(8):484-93.
Huang JJ,et al.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:MTOR

Research Areas:Epigenetics and Nuclear Signaling;Cancer?Cardiovascular?Cell biology;Metabolism