Phospho-Gli2 (Ser234) Antibody , Cat. # AF7072

SKU: AF7072

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:GLI2

GeneID:2736

Uniprot:P10070

Specificity:Phospho-Gli2 (Ser234) Antibody detects endogenous levels of Gli2 only when phosphorylated at Ser234.

Immunogen:A synthesized peptide derived from human Gli2 around the phosphorylation site of Ser234.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human

Prediction:Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Application:WB,ELISA(peptide)

Dilution:WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2843512

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF7072-BP

Des# Blocking Peptide:Phospho-Gli2 (Ser234) Blocking peptide

Blocking Peptide Price:$549 CAD