GeneBio Systems

PGE2 ELISA kit (General species)

Name: PGE2 ELISA kit (General species)
Brand: GeneBio Systems
SKU: CEA538Ge
Price: 972.00 GBP
Availability: InStock

SKU:CEA538Ge

£972.00 GBP

Sale Sold out

Shipping calculated at checkout.

Quantity

Size: 96Tests

# of Times Cited in literature: 85

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: PGE2

Target Full Name: Prostaglandin E2

Alternative Names: PG-E2

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species PGE2 / Prostaglandin E2

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 24.69-2,000pg/mL

Sensitivity: 8.67pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Prostaglandin E2 (PGE2). No significant cross-reactivity or interference between Prostaglandin E2 (PGE2) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGE2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGE2 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGE2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGE2 in the sample.

Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

References Citing This Product: Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid

Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis

Lipid mediators in plasma of autism spectrum disorders

Interleulin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage-dominant Microenvironment in Lung Cancer

Artesunate exerts an anti-immunosuppressive effect on cervical cancer by inhibiting PGE2 production and Foxp3 expression.

Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.

Celecoxib attenuates hepatic cirrhosis through inhibition of epithelial-to-mesenchymal transition of hepatocytes

Valproic acid protects septic mice from renal injury by reducing the inflammatory response

Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway.

Somatostatin prevents lipopolysaccharide

Celecoxib enhanced the cytotoxic effect of cisplatin in drug-resistant human gastric cancer cells by inhibition of cyclooxygenase-2