OxLDL ELISA kit (Mouse)

SKU: CEA527Mu

Size: 96Tests

# of Times Cited in literature: 10

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: OxLDL

Target Full Name: Oxidized Low Density Lipoprotein

Alternative Names: OL

Target Species: Mouse

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Mouse OxLDL / Oxidized Low Density Lipoprotein

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 2.47-200ng/mL

Sensitivity: 0.86ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxidized Low Density Lipoprotein (OxLDL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxidized Low Density Lipoprotein (OxLDL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Oxidized Low Density Lipoprotein (OxLDL). No significant cross-reactivity or interference between Oxidized Low Density Lipoprotein (OxLDL) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxidized Low Density Lipoprotein (OxLDL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxidized Low Density Lipoprotein (OxLDL) and unlabeled Oxidized Low Density Lipoprotein (OxLDL) (Standards or samples) with the pre-coated antibody specific to Oxidized Low Density Lipoprotein (OxLDL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxidized Low Density Lipoprotein (OxLDL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxidized Low Density Lipoprotein (OxLDL) in the sample.

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;

References Citing This Product: Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen

Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice.

Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice

Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western …

Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties

Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet

Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice

三七总皂苷对动脉粥样硬化小鼠的治疗作用

Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent

Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells

Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice

DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis