GeneBio Systems

OPG ELISA kit (Rat)

Name: OPG ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEA108Ra
Price: 835.00 GBP
Availability: InStock

SKU:SEA108Ra

£835.00 GBP

Sale Sold out

Shipping calculated at checkout.

Quantity

Size: 96Tests

# of Times Cited in literature: 25

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: OPG

Target Full Name: Osteoprotegerin

Alternative Names: TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

Target Species: Rat

Uniprot: O08727

Gene ID: 25341

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat OPG / Osteoprotegerin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 31.2-2,000pg/mL

Sensitivity: 15.3pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoprotegerin (OPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Osteoprotegerin (OPG). No significant cross-reactivity or interference between Osteoprotegerin (OPG) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteoprotegerin (OPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteoprotegerin (OPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteoprotegerin (OPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteoprotegerin (OPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Metabolic pathway;Bone metabolism;

References Citing This Product: Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy

Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar rats

The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model

Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells

The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.

Effect of Nicotine on RANKL and OPG and Bone Mineral Density

Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats

A novel model of bisphosphonate-related osteonecrosis of the jaw in rats

Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy

Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway

The effects of strength training and raloxifene on bone health in aging ovariectomized rats