OB ELISA kit (Rat)

SKU: CEC039Ra

Size: 96Tests

# of Times Cited in literature: -

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: OB

Target Full Name: Obestatin

Alternative Names: -

Target Species: Rat

Uniprot: P50596

Gene ID: 25608

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Rat OB / Obestatin

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 61.7-5,000pg/mL

Sensitivity: 24.7pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Obestatin (OB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Obestatin (OB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Obestatin (OB). No significant cross-reactivity or interference between Obestatin (OB) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Obestatin (OB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Obestatin (OB) and unlabeled Obestatin (OB) (Standards or samples) with the pre-coated antibody specific to Obestatin (OB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Obestatin (OB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Obestatin (OB) in the sample.

Research Area: Metabolic pathway;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;

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