NAADP(Nicotinic Acid Adenine Dinucleotide Phosphate) ELISA Kit

SKU: ELK8020

Size:96T

Reactivity:General

Alternative Names:NAADP

Assay Type:Competitive Inhibition

Sensitivity:51 ng/mL

Standard:10000 ng/mL

Detection range:156.25-10000 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NAADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NAADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NAADP in the samples is then determined by comparing the OD of the samples to the standard curve.

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