Mouse Ubiquitin-like Protein Isg15, ISG15 ELISA Kit

SKU: E2552Mo

Size:96 Tests-1 Kit

Background:Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response, IRF3 which inhibits its ubiquitination and degradation as well as EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL (By similarity).

Research_area:Cell Biology

Applications:ELISA

Target_protein:ISG15

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.053ng/mL

Detection range:0.1-40ng/mL

Sample type:Serum, plasma or other biological fluids

Full product name:Ubiquitin-like protein ISG15

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q64339

MASS(Da):17,898

GeneID:9636

Synonyms:G1P2;hUCRP;hUCRPIP17;IFI15;IMD38;Interferon-induced 15 kDa protein;Interferon-induced 17 kDa protein;IP17;ISG15;Ubiquitin cross-reactive protein;Ubiquitin-like protein ISG15;UCRP

Gene names:Isg15

Standard solution:48ng/mL