Mouse Toll-like Receptor 4, TLR-4 ELISA Kit

SKU: E1663Mo

Size:96 Tests-1 Kit

Background:Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed: 9851930, PubMed: 9989976, PubMed: 20133493). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed: 24380872). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (By similarity).

Research_area:Immunology; Microbiology; Cardiovascular

Applications:ELISA

Target_protein:TLR4

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.023ng/ml

Detection range:0.05-20ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Toll-Like Receptor 4

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9QUK6

MASS(Da):95,519

GeneID:21898

Synonyms:CD antigen CD284;TLR 4;TLR4;Toll-like receptor 4

Gene names:TLR4

Standard solution:24ng/ml