Mouse T3(Triiodothyronine) ELISA Kit

SKU: ELK10141

Size:96T

Reactivity:Mouse

Alternative Names:3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Assay Type:Competitive Inhibition

Sensitivity:0.12 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2.5h

Research Area:Endocrinology;Autoimmunity;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: