Mouse PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit

SKU: ELK5532

Size:96T

Reactivity:Mouse

Alternative Names:P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Assay Type:Competitive Inhibition

Sensitivity:1.45 ng/mL

Standard:300 ng/mL

Detection range:4.69-300 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Infection immunity;Bone metabolism;Rheumatology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIICP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: