Mouse NTS(Neurotensin) ELISA Kit

SKU: ELK0079

Size:96T

Reactivity:Mouse

Alternative Names:NEUT; neuromedin N; NN; NT; NT/N; NTS1; NMN-125

Assay Type:Competitive Inhibition

Sensitivity:12.2 pg/mL

Standard:3000 pg/mL

Detection range:46.88-3000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NTS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NTS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NTS in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9D3P9