Mouse NPAS4(Neuronal PAS Domain Protein 4) Microsample ELISA Kit

SKU: ELK7294MS

Size:96T

Reactivity:Mouse

Alternative Names:PASD10; NXF; Le-PAS; bHLHe79; Class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10

Assay Type:Sandwich

Sensitivity:0.127 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids.

Assay length:3.5h

Research Area:Neuro science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NPAS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPAS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NPAS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPAS4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q8BGD7