Mouse NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit

SKU: ELK9154

Size:96T

Reactivity:Mouse

Alternative Names:NF kappa B p65delta3; NFKB3

Assay Type:Sandwich

Sensitivity:12.2 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma, tissue homogenates

Assay length:3.5h

Research Area:Immunology

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q04207