Mouse F8(Coagulation Factor VIII) Microsample ELISA Kit

SKU: ELK10479MS

Size:96T

Reactivity:Mouse

Alternative Names:FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein

Assay Type:Sandwich

Sensitivity:0.081 IU/mL

Standard:20 IU/mL

Detection range:0.32-20 IU/mL

Sample type:plasma

Assay length:3.5h

Research Area:Metabolic pathway;Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F8 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q06194