Mouse Endonuclease 8-like 3, NEIL3 ELISA Kit

SKU: E2049Mo

Size:96 Tests-1 Kit

Background:DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA) (PubMed:20185759, PubMed:22065741, PubMed:22569481, PubMed:22959434, PubMed:23305905, PubMed:23313161). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases (By similarity). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA (PubMed:20185759, PubMed:22065741, PubMed:22569481, PubMed:22959434, PubMed:23305905, PubMed:23313161). No activity on 8-oxoG detected (PubMed:20185759, PubMed:22065741, PubMed:22569481, PubMed:22959434, PubMed:23305905, PubMed:23313161). Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity (PubMed:20185759, PubMed:22065741, PubMed:22569481, PubMed:22959434, PubMed:23305905, PubMed:23313161). In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues (PubMed:20185759, PubMed:22065741, PubMed:22569481, PubMed:22959434, PubMed:23305905, PubMed:23313161).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:NEIL3

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:9.92ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Endonuclease 8-like 3

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q8K203

MASS(Da):67,416

GeneID:234258

Synonyms:AI449477;BC034753;C85903;DNA glycosylase FPG2;DNA glycosylase/AP lyase Neil3;Endonuclease 8-like 3;Endonuclease VIII-like 3;NEIL3;Nei-like protein 3;

Gene names:Neil3

Standard solution:4800ng/L