Mouse ECM1(Extracellular Matrix Protein 1) Microsample ELISA Kit

SKU: ELK6591MS

Size:96T

Reactivity:Mouse

Alternative Names:Secretory component p85

Assay Type:Sandwich

Sensitivity:14.72 pg/mL

Standard:3000 pg/mL

Detection range:46.88-3000 pg/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Tumor immunity;Infection immunity;Immune molecule;Dermatology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ECM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ECM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ECM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ECM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q61508