Mouse Autophagy related protein 3,ATG3 ELISA KIT

SKU: E2634Mo

Size:96 Tests-1 Kit

Background:E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. {ECO:0000269|PubMed:11825910, ECO:0000269|PubMed:18768753, ECO:0000269|PubMed:20723759, ECO:0000269|PubMed:24089205}.

Research_area:

Applications:ELISA

Target_protein:Atg3

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.014ng/ml

Detection range:0.05-10ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Autophagy related protein 3

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9CPX6

MASS(Da):35796

GeneID:67841

Synonyms:Atg3 Apg3l

Gene names:Atg3

Standard solution:12.8ng/ml