Mouse Aromatase, ARO ELISA Kit

SKU: E1861Mo

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Research_area:Metabolism

Applications:ELISA

Target_protein:CYP19A1

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:1.48ng/L

Detection range:3-900ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Aromatase

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P28649

MASS(Da):58,015

GeneID:13075

Synonyms:Aromatase;CYP19A1;CYPXIX;Cytochrome P450 19A1;Cytochrome P-450AROM;Estrogen Synthase

Gene names:CYP19A1

Standard solution:960ng/L