GeneBio Systems
SKU:AQ621-01
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The product contains PerfectStart® Fast Taq DNA Polymerase, optimized dual-cation buffer, Influor Green I, dNTPs, PCR enhancer, PCR stabilizer and tracer dye. Three monoclonal antibodies bind to the FastTaq DNA Polymerase engineered by directed evolution with high affinity, which are prepared by optimized process, effectively blocking DNA polymerase activity and inhibiting non-specific amplification at low temperature. The qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, Universal Passive Reference Dye (optional) and nuclease-free water. This product uses the principle of mixing yellow and blue to generate green color to track the pipetting process. The qPCR SuperMix contains a yellow dye, and 10×Sample Buffer contains a blue dye. Adding template diluted in Sample Buffer with a brighter color (bright blue) to the qPCR SuperMix (light yellow) will show an obvious color change from yellow to green with easy naked-eye visualization which is more effective in avoiding pipetting errors.
•Traffic-light pipetting system: Blue qPCR master mix combines with samples prepared in a yellow dilution buffer to produce a green reaction mix, providing a clear visual indication of pipetting progress. This color-coded system helps operators track PCR setup across 96-well plates, reducing pipetting errors, missed wells, and costly sample mix-ups.
• PerfectStart® Fast Taq DNA Polymerase: fast and efficient; hot-start and blocking by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.
• Dual-cation buffer enhances specificity and reduces primer-dimer formation.
• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to slight variations in reaction volume or differences in well position.
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Component |
AQ621-01 | AQ621-02 | AQ621-03 | AQ621-04 |
| 2×PerfectStart® Visual Green qPCR SuperMix | 1 ml | 5×1 ml | 15×1 ml | 25×1 ml |
| 10×Sample Buffer | 200 μl | 1 ml | 3×1 ml | 5×1 ml |
| Universal Passive Reference Dye (50×) | 40 μl | 200 μl |
600 μl | 1 ml |
| Nuclease-free Water | 1 ml | 5 ml | 3×5 ml | 5×5 ml |
