MBP CLIA kit (Human)

SKU: SCA539Hu

Size: 96Tests

# of Times Cited in literature: -

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: MBP

Target Full Name: Myelin Basic Protein

Alternative Names: Myelin A1 protein; Myelin membrane encephalitogenic protein

Target Species: Human

Uniprot: P02686

Gene ID: 4155

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human MBP / Myelin Basic Protein

Method: Chemiluminescence

Detection principle: Double-antibody Sandwich

Detection range: 41.2-30,000pg/mL

Sensitivity: 17.5pg/mL

Assay Time: 2h, 40min

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Cerebrospinal Fluid and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP). No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myelin Basic Protein (MBP) level in the sample or standard.;

Research Area: Infection immunity;Neuro science;Autoimmunity;

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