LPC ELISA kit (General species)

SKU: CEK621Ge

Size: 96Tests

# of Times Cited in literature: 8

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: LPC

Target Full Name: Lysophosphatidylcholine

Alternative Names: lysoPC; Lysolecithin; 1-Palmitoyl-sn-Glycero-3-Phosphocholine

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species LPC / Lysophosphatidylcholine

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 78.125-20,000ng/mL

Sensitivity: 27.142ng/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine (LPC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine (LPC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine (LPC). No significant cross-reactivity or interference between Lysophosphatidylcholine (LPC) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lysophosphatidylcholine (LPC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lysophosphatidylcholine (LPC) and unlabeled Lysophosphatidylcholine (LPC) (Standards or samples) with the pre-coated antibody specific to Lysophosphatidylcholine (LPC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lysophosphatidylcholine (LPC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lysophosphatidylcholine (LPC) in the sample.

Research Area: -

References Citing This Product: High-fat diet-induced acceleration of osteoarthritis is associated with a distinct and sustained plasma metabolite signature

Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids‐Epigenetic Circuit

Associations between plasma lysophospholipids concentrations, chronic kidney disease and the type of renal replacement therapy

ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy

Dietary Betaine Addition Promotes Hepatic Cholesterol Synthesis, Bile Acid Conversion, and Export in Rats

iPLA2¦ Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury

Downregulation of activating transcription factor 4 attenuates lysophosphatidycholine-induced inflammation via the NF-κB pathway

Astragaloside IV Inhibits Bleomycin-Induced Ferroptosis in Human Umbilical Vein Endothelial Cells by Mediating LPC