LOX1 ELISA kit (Human)

SKU: SEB859Hu

Size: 96Tests

# of Times Cited in literature: 13

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: LOX1

Target Full Name: Lectin Like Oxidized Low Density Lipoprotein Receptor 1

Alternative Names: OLR1; CLEC8A; SCARE1; Oxidized low-density lipoprotein receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; Lectin-type oxidized LDL receptor 1

Target Species: Human

Uniprot: P78380

Gene ID: 4973

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human LOX1 / Lectin Like Oxidized Low Density Lipoprotein Receptor 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.060ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). No significant cross-reactivity or interference between Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Metabolic pathway;Apoptosis;Endocrinology;Cardiovascular biology;Neuro science;

References Citing This Product: Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels Are Associated With Angiographic Coronary Lesion Complexity in Patients With Coronary Artery Diseas

Postprocedural serum sLOX-1 levels are associated with coronary in-stent restenosis in patients with stable coronary artery disease

Soluble lectin-like oxidized low-density lipoprotein receptor-1 levels in synovial fluid are correlated with disease severity of knee osteoarthritis

Pregnancy Followed by Delivery May Affect Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels in Women of Reproductive Age

Lectin-like oxidized low density lipoprotein receptor 1(LOX-1) levels and endothelial dysfunction in patients with primary essential hyperhidrosis/Primer esansiyel hiperhidroz hastalarinda lektin benzeri okside düsük dansiteli lipoprotein reseptor 1(LOX-1) düzeyleri ve endotel disfonksiyonu

Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome

Endothelial damage in white coat hypertension: role of lectin-like oxidized low-density lipoprotein-1