LH ELISA kit (Human)

SKU: CEA441Hu

Size: 96Tests

# of Times Cited in literature: 8

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: LH

Target Full Name: Luteinizing Hormone

Alternative Names: ICSH; Lutropin; Interstitial Cell Stimulating Hormone

Target Species: Human

Uniprot: P01215 & P01229

Gene ID: 1081 & 3972

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with Human LH / Luteinizing Hormone

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 1.05-85mIU/mL

Sensitivity: 0.41mIU/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH). No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone (LH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample.

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

References Citing This Product: Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis

Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.

New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.

Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells

Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression