IgG4 ELISA kit (Human)

SKU: SEA234Hu

Size: 96Tests

# of Times Cited in literature: 5

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IgG4

Target Full Name: Immunoglobulin G4

Alternative Names: IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

Target Species: Human

Uniprot: P01861

Gene ID: -

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human IgG4 / Immunoglobulin G4

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.074ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G4 (IgG4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G4 (IgG4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G4 (IgG4). No significant cross-reactivity or interference between Immunoglobulin G4 (IgG4) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G4 (IgG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G4 (IgG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G4 (IgG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G4 (IgG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;Immune molecule;

References Citing This Product: The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling

Utility of serum IgG, IgG4 and carbonic anhydrase II antibodies in distinguishing autoimmune pancreatitis from pancreatic cancer and chronic pancreatitis

Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer

Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells

Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s disease

The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling

Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patients