GeneBio Systems

IGF1 ELISA kit (Rat)

Name: IGF1 ELISA kit (Rat)
Brand: GeneBio Systems
SKU: SEA050Ra
Price: 571.00 GBP
Availability: InStock

SKU:SEA050Ra

£571.00 GBP

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Quantity

Size: 96Tests

# of Times Cited in literature: 26

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IGF1

Target Full Name: Insulin Like Growth Factor 1

Alternative Names: IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

Target Species: Rat

Uniprot: P08025

Gene ID: 24482

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat IGF1 / Insulin Like Growth Factor 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.065ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Endocrinology;

References Citing This Product: A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats

The vagus nerve as a regulator of growth hormone secretion

Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in Rats

Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress.

The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model

Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats

Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy

BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause

The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats

Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle