Human Ubiquitin Carboxyl Terminal Hydrolase L3,UCHL3 ELISA KIT

SKU: E6969Hu

Size:96 Tests-1 Kit

Background:Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. {ECO:0000269|PubMed:19154770, ECO:0000269|PubMed:21762696, ECO:0000269|PubMed:22689415, ECO:0000269|PubMed:2530630, ECO:0000269|PubMed:9790970}.

Research_area:

Applications:ELISA

Target_protein:UCHL3

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.016ng/ml

Detection range:0.05-10ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Ubiquitin Carboxyl Terminal Hydrolase L3

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P15374

MASS(Da):26183

GeneID:7347

Synonyms:UCHL3

Gene names:UCHL3

Standard solution:12.8ng/ml