Human Transcriptional Coactivator Yap1, YAP1 ELISA Kit

SKU: E6874Hu

Size:96 Tests-1 Kit

Background:Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750). Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Research_area:Signal Transduction; Epigenetics and Nuclear Signaling; Cancer

Applications:ELISA

Target_protein:YAP1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.093ng/ml

Detection range:0.2-60ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Transcriptional coactivator YAP1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P46937

MASS(Da):54,462

GeneID:10413

Synonyms:COB1;Protein yorkie homolog;Transcriptional coactivator YAP1;YAP;YAP1;YAP2;YAP65;Yes-associated protein 1;Yes-associated protein YAP65 homolog;YKI

Gene names:YAP1

Standard solution:64ng/ml