GeneBio Systems
Human RvD1(Resolvin D1) ELISA Kit
Human RvD1(Resolvin D1) ELISA Kit
SKU:ELK0604
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Size:96T
Reactivity:Human
Alternative Names:RvD1
Assay Type:Competitive Inhibition
Sensitivity:5.68 pg/mL
Standard:1000 pg/mL
Detection range:15.63-1000 pg/mL
Sample type:serum, plasma and other biological fluids
Assay length:2.5h
Research Area:Immunology & Inflammation
Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human RvD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RvD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RvD1 in the samples is then determined by comparing the OD of the samples to the standard curve.
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