Human Protein Lin-28 Homolog B, LIN28B ELISA Kit

SKU: E0441Hu

Size:96 Tests-1 Kit

Background:Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed: 22118463). Does not act on pri-miR21 (PubMed: 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed: 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed: 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Research_area:Others

Applications:ELISA

Target_protein:LIN28B

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.044ng/ml

Detection range:0.1-35ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Protein Lin-28 Homolog B

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q6ZN17

MASS(Da):27,084

GeneID:389421

Synonyms:LIN28B;Lin-28B;Protein lin-28 homolog B

Gene names:LIN28B

Standard solution:40ng/ml