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GeneBio Systems

Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) Microsample ELISA Kit

Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) Microsample ELISA Kit

ELK8570MS

£465.00 GBP
£465.00 GBP
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Size:96T

Reactivity:Human

Alternative Names:PIVKA-II; PIVKA II

Assay Type:Sandwich

Sensitivity:0.13 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Hematology

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-II. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIVKA-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIVKA-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIVKA-II in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:

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