Human PC(Protein Carbonyl) Microsample ELISA Kit

SKU: ELK9275MS

Size:96T

Reactivity:Human

Alternative Names:Protein Carbonyl

Assay Type:Sandwich

Sensitivity:0.122 nmol/mL

Standard:20 nmol/mL

Detection range:0.32-20 nmol/mL

Sample type:Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Lysate

Assay length:3.5h

Research Area:Metabolic pathway;Hepatology;Hormone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PC in the samples is then determined by comparing the OD of the samples to the standard curve.

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