Human Mitochondrial Cardiolipin Hydrolase, PLD6 ELISA Kit

SKU: E2645Hu

Size:96 Tests-1 Kit

Background:Endonuclease that plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. piRNAs provide essential protection against the activity of mobile genetic elements (By similarity). piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface (By similarity). Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol (PubMed:21397848). Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity. Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Also acts as a regulator of mitochondrial shape through facilitating mitochondrial fusion (PubMed:17028579, PubMed:26711011).

Research_area:Others

Applications:ELISA

Target_protein:PLD6

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.045ng/ml

Detection range:0.1-38ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Mitochondrial cardiolipin hydrolase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q8N2A8

MASS(Da):28,273

GeneID:201164

Synonyms:Choline phosphatase 6;Mitochondrial cardiolipin hydrolase;Mitochondrial phospholipase;MitoPLD;Phosphatidylcholine-hydrolyzing phospholipase D6;Phospholipase D6;PLD 6;PLD6;Protein zucchini homolog;ZUC

Gene names:PLD6

Standard solution:40ng/ml